|
KMID : 0381219780100020031
|
|
Journal of RIMSK
1978 Volume.10 No. 2 p.31 ~ p.38
|
|
|
Experimental Study on the Effects of Immune Mechanism on the Regeneration of Ovarian Transplants
|
|
|
|
|
Abstract
|
|
|
Since Biskind and Biskind (1944) had adopted a method of ovarian transplantation into the spleen of the castrated rats to induce ovarian tumor, it was confirmed by many authors as aa method of artificial tumor induction. Park and Kim were first authors who ascertained the role of interstitial gland, which had been found by Touneux at 1889, during the regeneration and tumor formation of ovarian transplants.
Biskind and Biskind (1949) reported the serial changes of the ovarian transplants up to the time (day) of tumor formation as follows; necrosis at 2nd day of transplantation, inflammatory cell infiltration at 8th day, reappearance of primordial follicle at 10th day, reappearance of corpus luteum at 13th day, occurrence of luteoma at 157th day, and occurrence of granulosa cell tumor at 317th day.
However, Park and Kim reported some differences in tumor formation in mice. It were as follows; edematous changes at 24th hour of transplantation, necrosis at 2nd day, regeneration of primordial follicle at 5th day, reappearance and hyperplasia of the interstitial glands at 92th day, occurrence of luteoma at 169th day, occurrence of granulosa cell tumor at 232th day and occurence of anaplastic changes of granulosa cell tumor and occurrence of thecoma at 310th day. But they reported that they could not find any corpus luteum during the regeneration of the ovarian transplants. Such findings were confirmed by many authors.
Greenwald and Guraya reported that ~there were some relationships between the amounts and kinds of the ovarian lipid and maturation ;of corpus luteum. And Park (1977) reported that the interstitial glands of the ovary were regenerated and active functionally after 110th
days of ovarian transplantation.
It is generally accepted that the homograft has short life-span and is usually easily rejected than the autograft(Park and Good).
In order to clarify the difference between reports of Biskind and Biskind (1949) and reports of Park and Kim (1973) and the diffects.of immune mechanism between autograft and homograft, the author transplanted the ovaries into itself´s spleen (autograft) and other´s spleen (homograft). After transplantation, the experimental animals were sacrified at 1st, 2nd, 4th, 8th, 16th and 32th day of transplantation. The ovarian transplants were kept in the refrigator at -25°C and serially sectioned at 8 micron in the thickness. And several lipid stainings were performed and the following results were ensued;
1. The ovarian tissue transplanted ?into the spleen of the castrated rats were regenerated after necrosis in situ.
2. There was no significant differences between in the group of autotransplantation and in the group of homotransplantation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|